Research Interest: Bacteria of the genus Staphylococcus, in particular the species S. aureus and virulence factors produced by this species. In the so far carried out research, the results of which were published in several peer-reviewed journals I analyzed:

1.The influence of leucocytotoxic and superantygenic virulence factors produced by S. aureus strains isolated from milk of cows with mastitis on the cytokine production and gene expression in bovine leukocyte cultures in vitro;
2. the impact of staphylococcal exotoxins on the process of phagocytosis in vitro;
3. the efficacy of treatment of mastitis by staphylococcal autovaccine;
4. the profile of resistance to methicillin of coagulase-negative staphylococci isolated from animals;
5. the profile of the genes encoding superantigens in S. aureus and S. xylosus isolates collected from cows;
6. the possibility of transfer of genes encoding superantigens between staphylococci occurring in the same mammary gland;
7. genetic relatedness of staphylococci isolated within a herd of animals;
8. the biological activity of staphylococcal exosecretions against leukocytes in vitro;
9. the impact of staphylococcal virulence factors on the morphology of leukocytes in vitro.
I also analyzed and published in the GenBank several new 16S rRNA gene sequences of bacteria representing different species of staphylococci, identified by PCR-RFLP analysis of gap gene. Furthermore, as a contributor I published in the GenBank the 16S rRNA gene sequences of bacterial isolates, which were unidentified based on the polymorphism of gap gene, of which at least two isolates represent new species or subspecies of the staphylococci.
The second direction of my research concerns the use of magnetic fields, in particular the rotating magnetic field to modify the functional parameters and pathogenicity potential of different species of microorganisms. At the present, I’m analyzing the influence of magnetic field on metabolic processes in microorganisms, both at the cellular and molecular level. As a continuation of my first research mainstream microorganism used in these studies is primarily S. aureus.

Research Interest: Immune based inflammatory disorders (with special reference to nanomedicines).

I am a Postdoctoral Research Scientist at the Columbia University Irving Medical Center where I am understanding the mechanism of AICDA, a DNA mutator with paramount importance in antibody diversity in on target activity using human Bcells. I am studying this by targeted degradation of essential proteins using a state of art technology called Degron system which are generated using knockin and single cell cloning. These targets were identified from mass spec and mutations seen in DHL and DLBCL a form of Bcell lymphoma.

During my Ph.D I delineated interaction between CD40L- CD40 during a macrophages T cell interaction using residue specific functions on CD40L which are reported in hyper IgM syndrome. While studying this I also generated avirulent strains of Leishmania parasites which we testes as a vaccine strain in different knockout mice and studied the immune response elicited by this vaccine. I also developed a DNA vaccine against one of the target proteins in the Leishmania and testes it. During my master's in Virology from the National Institute of Virology, Pune I study different classes of virus and their pathogenesis. In particular, I studied the role of host kinases in the pathogenesis of the Japanese Encephalitis virus for my master's dissertation. I have a deep understanding of Immunology, APC-Tcell interaction, host-pathogen interactions, RNA biology, Bcell biology and vaccines. My area of interest is immunology, tumor immunology, macrophages, Bcells, host-pathogen interactions, virology, RNA biology, and cancer biology.

Research Interest: Lymphoma, Bcell biology, receptor-ligand interaction, host-pathogen-interaction, macrophages and tumor immunity

Research Interest: Antigen processing and presentation, relating to the understanding of these processes and manipulation of them in order to treat.

Research Interest: Cancer immunology, vaccines, cancer, antigen-presenting cells and viral immunology.

Research Interest: Endocrine and metabolic diseases in prostate cancer, bone metabolism, renal function, and immune structure in prostate cancer.

Research Interest: Genitourinary Cancer

Research Interest: Veterinary Vaccine and Immunology, Veterinay Virology (Canine distemper virus, Canine parvovorus and Classical swine fever virus.

Research Interest: Biotechnology, Vaccines, Vaccine Development, Virology, Viral Vaccines.

Research Interest: Vaccine, innate immunity, parasite-host interaction.

Since July 2008, assistant in the Department of Epidemiology, MU- Plovdiv

From April 2020 epidemiologist at the Hygiene and Epidemiology Department, University Hospital "St. George" EAD

From September 2022 Acting Head of the Hygiene and Epidemiological Department, University Hospital "St. George" EAD

On 27.12.2020 applied the first vaccine in the history of the vaccination campaign against COVID-19 in Plovdiv

In 2021, she was part of the Ministry of Education and Culture of the Republic of Bulgaria campaign to educate teachers about vaccines against COVID-19.

In 2021-2022 advises on vaccines againstCOVID-19 on the National Patient Helpline toll-free Organization.

2021-2022 cooperates with the foundation "For our children"

2021-2023 participated in expert meetings on epidemiology at the Ministry of Health of the Republic of Bulgaria.

In 2022,  she was part of a working group at the Council of Ministers- Republic of Bulgaria.

Member of:

International epidemiological association (IEA) Bacti Vac Network. Bulgarian Scientific Society for Epidemiology of Infectious and Non-Infectious Diseases Bulgarian Association of Preventive Medicine. The South Bulgarian Society of Infectious Diseases, Epidemiology and Parasitology. Bulgarian Association for Prevention and Infection Control.

Research Interests:

Vaccines, vaccinology, infectious diseases, emerging diseases, epidemics, pandemics, COVID-19, varicella, shingles, measles.

List of Publications:

1. Tomov L, Chervenkov L, Miteva DG, Batselova H, Velikova T. Applications of time series analysis in epidemiology: Literature review and our experience during COVID-19 pandemic. World J Clin Cases. 2023 Oct 16;11(29):6974-6983. doi: 10.12998/wjcc.v11.i29.6974. PMID: 37946767; PMCID: PMC10631421.
   
2. Mileva N, Vasilev GH, Ganev B, Chervenkov L, Batselova H, Tzotcheva I, Tomov L, Velikova T, Lazova S. Cardiovascular Manifestations of Multisystem Inflammatory Syndrome in Children: A Single-Center Bulgarian Study. Medicina (Kaunas). 2023 Dec 14;59(12):2175. doi: 10.3390/medicina59122175. PMID: 38138278; PMCID: PMC10744581.
3. Dimitrov, G., Valkov, T., Batselova, H., Kounchev, O., Momekov, G., Argirova, R. Nationwide analysis of the impact of COVID-19 in patients with a cardiovascular, oncological or chronic pulmonary disease in the context of an Eastern European country with a low vaccination rate, Bulgaria: March 2020-April 2022 (2023) BMJ Open, 13 (8), art. no. e068431,
4. Miteva, D., Kitanova, M., Batselova, H., Lazova, S., Chervenkov, L., Peshevska-Sekulovska, M., Sekulovski, M., Gulinac, M., Vasilev, G.V., Tomov, L., Velikova, T. The End or a New Era of Development of SARS-CoV-2 Virus: Genetic Variants Responsible for Severe COVID-19 and Clinical Efficacy of the Most Commonly Used Vaccines in Clinical Practice (2023) Vaccines, 11 (7), art. no. 1181.
5. Ivanov N, Krastev B, Miteva DG, Batselova H, Alexandrova R, Velikova T. Effectiveness and safety of COVID-19 vaccines in patients with oncological diseases: State-of-the-art. World J Clin Oncol. 2023 Sep 24;14(9):343-356
6. Tomov, L., Batselova, H., Lazova, S., Ganev, B., Tzocheva, I., Velikova, T. Role of children in the Bulgarian COVID-19 epidemic: A mathematical model study (2023) World Journal of Experimental Medicine, 13 (3), pp. 28-46.
7.  Sekulovski, M. Mileva, N. Vasilev, G.V. Miteva, D. Gulinac, M. Peshevska-Sekulovska, M. Chervenkov, L. Batselova, H. Vasilev, G.H.; Tomov, L.; et al. Blood Coagulation and Thrombotic Disorders following SARS-CoV-2 Infection and COVID-19 Vaccination. Biomedicines 2023, 11, 2813.
8. Slabakova, Y.; Gerasoudis, S.; Miteva, D.; Peshevska-Sekulovska, M.; Batselova, H.; Snegarova, V.; Vasilev, G.V.; Vasilev, G.H.; Sekulovski, M.; Lazova, S.; et al. SARS-CoV-2 Variant-Specific Gastrointestinal Symptoms of COVID-19: 2023 Update. Gastroenterol. Insights 2023, 14, 431-445. https://doi.org/10.3390/gastroent14040032 .
9. Tomov, L.P., Batselova, H., Velikova, T.Changes in Patterns of Infectivity and Mortality with SARS-CoV-2 Omicron Variant in Bulgaria.Computer Science and Education in Computer Science. CSECS 2023. Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering, vol 514. Springer, Cham. https://doi.org/10.1007/978-3-031-44668-9_15.
10. Tomov, L.P., Batselova, H.M., Velikova, T.V. COVID-19 Delta Wave Caused Early Overburden of Hospital Capacity in the Bulgarian Healthcare System in 2021 (2022) Healthcare (Switzerland), 10 (4), art. no. 600.
11. Tomov, L., Batselova, H., Velikova, T.V. Estimating COVID Case Fatality Rate in Bulgaria for 2020–2021(2022) Lecture Notes of the Institute for Computer Sciences, Social-Informatics and Telecommunications Engineering, LNICST, 450 LNICST, pp. 102-115.
12. Snegarova V, Miteva D, Gulinac M, Peshevska-Sekulovska M, Batselova H, Velikova T. COVID-19 inpatients with gastrointestinal stromal tumors: Recommendations for management and vaccination. World J Gastrointest Pathophysiol 2022; 13(5): 170-177
13. Vasilev G, Kabakchieva P, Miteva D, Batselova H, Velikova T. Effectiveness and safety of COVID-19vaccines in patients with diabetes as a factor for vaccine hesitancy. World J Diabetes 2022; 13(9): 738-751.
14. Miteva D, Peshevska-Sekulovska M, Snegarova V, Batselova H, Alexandrova R, Velikova T. Mucosal COVID-19 vaccines: Risks, benefits and control of the pandemic. World J Virol 2022; 11(5): 221-236.
15. Tomov L, Miteva D, Sekulovski M, Batselova H, Velikova T. Pandemic control-do's and don'ts from a control theory perspective. World Journal of Methodology 12.5 (2022): 392.
16. Velikova, T., Kukov, A., Mihova, A., Snegarova, V., Batselova, H., Nakov, R. Gastrointestinal mucosal immunity and covid-19 (2021) World Journal of Gastroenterology, 27 (30), pp. 5047-5059.
17. Tomov, L., Velikova, T., Batselova, H. HEALTH RISK MANAGEMENT IN KINDERGARTENS AND SCHOOLS DURING COVID-19 PANDEMIC (2021) General Medicine, 23 (6), pp. 22-27.
    
18. Velikova, T., Batselova, H., Jenkov, V. IMPACT OF COVID‐19 PANDEMIC ON MENTAL HEALTH: A CROSS‐SECTIONAL SURVEY OF 1,020 BULGARIAN CITIZENS. Bulgarian Journal of Psychiatry (2020), 5 (3) , 10-18.
19. Velikova TV, Kotsev SV, Georgiev DS, Batselova HM. Immunological aspects of COVID-19: What do we know? World J Biol Chem. 2020 Sep 27;11(2):14-29.
20. Бацелова Х., Кеворкян А., Райчева Р., Бойкинова О., Балтаджиев И., Шопова Й., Стоилова Й. Епидемиологично проучване на хоспитализираните пациенти с варицела в Пловдивска област за периода 2000-2011г. Обща медицина, 2013, XV (3).
21. Baltadzhiev, I., Stoilova, J., Popivanova, N., Bazelova, H. Changes in the Viral Hepatitis B (VHB) morbidity 1987-2006 (2009) Problems of Infectious and Parasitic Diseases, 37 (1), pp. 39-40. 

Research Interest: Pathogenesis and Treatment of Melanoma, and in Part are directed at Developing Tools to Study Melanoma.

Research Interest: Gene therapy, Cell and tissue engineering, Vaccine development, Biomaterials, Synthetic biology and biomass, Nanobiotechnology.

Research Interest: Allergy, Allergens, Carbohydrates, cross-reactive carbohydrate deteminants
Immunomodulation, Antibody engineering, Molecule design, Molecular biology and biochemistry
Recombinant technologies.